Journal: Journal of nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration.

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Fig. 1 Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 107 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: STAR protocols

Article Title: Enzyme-free isolation of mesenchymal stem cells from decidua basalis of the human placenta.

doi: 10.1016/j.xpro.2023.102498

Figure Lengend Snippet: Figure 2. Immunophenotypic characterization of isolated PL-MSCs The antibodies used were (A) human CD73, (B) human CD90 (C) human CD105 (D) human CD166 (E) human CD34 (F) human HLA-DR and their respective isotype controls. Open histogram indicates background signal, while, shaded histogram represents positive reactivity with the indicated antibodies. Table shows percentage of cells expressing the respective surface markers (n = 3) in mean G SEM.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE Mouse Anti-Human CD73 (1:33) BD Pharmingen Catalog No 550257 PE Mouse Anti-Human CD90 (1:33) BD Pharmingen Catalog No 555596 CD105 PE-conjugated Antibody (1:50) R&D Systems Catalog No FAB10971P PE Mouse Anti-Human CD34 (1:50) BD Pharmingen Catalog No 550761 PE Mouse Anti-Human CD166 (1:50) BD Pharmingen Catalog No 559263 FITC Mouse Anti-Human HLA-DR (1:33) BD Pharmingen Catalog No 555811 PE Mouse IgG, k Isotype Control (1:33 as isotype for CD90, 1:800 as isotype for CD34/ CD73/CD166) BD Pharmingen Catalog No 550617 Mouse IgG1 PE-conjugated Antibody (1:50) R&D Systems Catalog No IC002P FITC Mouse IgG2a, k Isotype Control (1:33) BD Pharmingen Catalog No 555573 Vimentin (D21H3) XP Rabbit mAb Antibody (1:150) Cell Signaling Technology Catalog No 5741S Human STRO-1 antibody (1:100) R&D Systems Catalog No MAB1038 Oct-4 (D705Z) Mouse mAb (1:400) Cell Signaling Technology Catalog No 75463S Alexa Fluor 488 goat anti-mouse (1:500) Invitrogen Catalog No A11001 Alexa Fluor 568 goat anti-rabbit (1:500) Invitrogen Catalog No A11011 Alexa Fluor 488 anti-rabbit (1:500) Cell Signaling Technology Catalog No 4412S Biological samples Human placenta N/A N/A Chemicals, peptides, and recombinant proteins 0.9% w/v Saline Kunal Remedies Pvt.

Techniques: Isolation, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Human placenta-derived mesenchymal stem cells stimulate neuronal regeneration by promoting axon growth and restoring neuronal activity

doi: 10.3389/fcell.2023.1328261

Figure Lengend Snippet: Characterization of hPMSCs. (A) Morphology at passage 3. Both normoxic and hypoxic cultures show highly homogeneous spindle-like shape. Scale bar: 500 µm (B) Proliferation capacity. Cumulative population doublings of hPMSCs grown under hypoxia (square) are higher than cultures grown in normoxia (dot). (C) Flow cytometry shows specific hMSCs marker expression pattern: positive for CD105, CD44, CD90, CD73 and negative for CD14, CD19, CD34, CD45 and HLA-DR. (D) Osteogenic differentiation was confirmed by Alizarin red staining of calcium deposits. (E) Adipogenesis differentiation was followed by Oil Red O staining of lipids vacuoles (black arrows indicate lipid droplets). (F) Alcian Blue staining of proteoglycans demonstrated chondrogenesis differentiation. Scale bar: 100 μm. Two-way ANOVA and post hoc Sidak test for multiple comparisons between means (** p ≤ 0.005; * p ≤ 0.05).

Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

Techniques: Flow Cytometry, Marker, Expressing, Staining